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OPTOGENETICS TOOLBOX        



Optogenetic-toolkit
  1 Ultra High Power LED   2 Beam Combiner
  3 Fiber Coupling Adaptor   4 Fiberoptics
  5 Rotary Joint   6 Beam Switcher
  7 Filter Wheel   8 Microscope Adaptors
  9 Liquid Light Guide Adaptor 10 Liquid Light Guide
11 Liquid Light Guide XYZ Collimator 12 C-Mount Adaptor
13 Fiberoptic Collimator 14 Reference Photodiode
15 Single/Dual Fiber 16 Optogenetics Implantable Cannulae

 Ultra High Power LEDs

(see [1] on drawing)
 
  Ultra High Power (UHP) LEDs are excellent light sources for Optogenetics experiments. The UHP LEDs feature ultra-high brightness at small active area which enables both fiber coupling applications and epifluorescence excitation.
The UHP LED driver is controlled by a fast opto-isolated TTL input for fast switching with a rise/fall time of microseconds, much faster than the millisecond pulses required for Optogenetics applications. For example the blue UHP-Mic-LED-460 is excellent for Channelrhodopsin activation which requires 460nm activation.

 Beam Combiner

 (see [2] on drawing)

  The Beam Combiner can join two discrete UHP-LEDs into one output beam, which can then be coupled to a microscope or an optical fiber. For increased versatility, additional optical filters can be installed at the input or the output ports of the Beam Combiner.

 Fiber Coupling Adaptor

  (see [3] on drawing)
Fiber-Coupled Adaptor   
  The UHP-LED can be easily changed from direct microscope coupling to fiber coupled LED configuration, using this Fiber Coupler Adaptor. This adaptor can be easily assembled by the user on the UHP-LED output.
For more details please see video clip

 Fiber Optics

  (see [4] on drawing)

  Prizmatix offers a wide range of standard and customized multi-mode silica / polymer fibers as well as ferrules for optogenetics research.
   A Y-shaped fiber bundle is especially useful, offering the option to activate two brain hemispheres simultaneously: a 1000μm or 1500μm fiber is coupled to the LED and connected to a rotary joint.
   From the joint’s other side emerge two thin fibers which can then be connected to two separate ferrules. The light from the LED is divided equally between the two hemispheres without any loss of power.


 Rotary Joint

  (see [5] on drawing)

  Prizmatix Rotary Joint is especially designed for Optogenetics experiments with High NA fibers equipped with FC connectors.

  Rotary Joint very low friction and smooth swivel operation are demonstrated in this video - showing continuous rotation for over 30 seconds after a fingertip startup.

  The torque required for free movement is very low thus reducing the Rotary Joint's behavioral effect on free moving animals.

  Prizmatix Rotary Joint can be used with large core input optical fiber connected to several thin output fibers simultaneously. This allows concurrent delivery of light to separate areas of the brain without loss of power due to the fiber split.  See more..

Rotary Joint PDF

      Fiber-Optic-Rotary-Joint

 Beam Switcher

  (see [6] on drawing)
Beam-Switcher   The Beam Switcher accessory allows for Prizmatix LEDs installed on a microscope to be used either as microscope epi-fluorescence illumination or illuminating a specimen via a fiberoptic probe.
  Especially useful for in-situ Optogenetics studies, the Beam Switcher saves cost, space and allows maximum flexibility in experiment design. See more..

Beam switcher PDF


 Filter Wheel

  (see [7] on drawing)
  The UHP-Mic-LED can be equipped with a 6 positions filter wheel at the beam output. This accessory is particularly useful for UHP-Mic-LED-White light source.  See more ...

Filter Wheel PDF

Filter-Wheel

 Microscope Adaptors

  (see [8] on drawing)
Microscope-Adaptor   Use these adaptors to connect Prizmatix LED light sources to the most widely used microscopes. Adaptors for epi-fluorescence ports of Nikon, Zeiss, Olympus and Leica microscopes are available.

 Liquid Light Guide Adaptor

  (see [9] on drawing)
  The UHP-LEDs can be easily changed from direct microscope coupling to Liquid Light Gide (LLG) coupled using this Adaptor. This adaptor can be without difficulty assembled by the user on the UHP-LED output. Please see video clip for more details.

 Liquid Light Guide

  (see [10] on drawing)
  A flexible 3mm core liquid light guide is ideal to conduct light from the large LED emitter to the microscope.


 Liquid Light Guide XYZ Collimator

  (see [11] on drawing)
LLG-Collimator   The Prizmatix LLG collimator is an XYZ adjustable collimator for Liquid Light Guide (LLG) with adaptor for the epi-illumination port of fluorescence microscopes. This collimator can be equipped with Nikon, Olympus, Zeiss or Leica adaptors.

 C-Mount adaptor

  (see [12] on drawing)
  A standard C-mount thread adaptor (1-32 UN 2A thread), usually used to connect to trinocular microscopes.

 Fiberoptic Collimator

  (see [13] on drawing)
  Collimators are required to generate a parallel beam of light out of the naturally diverging light emission from an optical fiber. Prizmatix collimator is designed to collimate High NA fibers (up to 0.53), such as Hard Polymer Clad fibers and Polymer Optical Fibers (POFs).

Collimator PDF

collimator

 Reference Photodiode

  (see [14] on drawing)

  Prizmatix reference photodiode is useful in order to monitor LED source power over time in experiments required exceptional stability over long time period.
  The photodiode signal can be used to normalize measurements; alternatively the photodiode signal can serve as a feedback signal to stabilize the LED power.



 Single/Dual Fiber

  (see [15] on drawimg)
  Prizmatix provides a full solution to the optogenetics in vivo and in vitro fiber optics. Made of silica or polymer fibers, the high NA fibers are assembled to fit any research set-up with various combinations of connectors, ferrules, core diameters and lengths. Some examples are Y-shaped fibers for simultaneous in-vivo stimulation of two hemispheres, and fibers with stainless steel tip with protruding bare fibers for in-vitro activation of brain slices under a microscope.

See more ...
Optogenetics-Protruding-Fiber-tip

 Optogenetics Implantable Cannulae

  (see [16] on drawing


  Prizmatix Implantable Fiberoptic Cannulae / Ferrules allow direct light stimulation of certain deep brain regions in living animals.   Each Cannula consists of a Zirconia ferrule accommodating a high NA Silica optical fiber, protruding from the Zirconia ferrule at a desired length. See more...

Cannula PDF


 

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